RayPlex Porcine Inflammation Bead Array 1(FAP-INF-1)

规格

规格	192 tests、48 tests、480 tests、96 tests
样本类型	Cell Culture Supernatants, Serum
原理方法	Sandwich-based
检测方法	Flow Cytometry
定量与否	Quantitative
载体	Fluorescent Microbeads
适用种属	Porcine (pig)
检测靶标	IL-6 / IL6, IL-1 beta / IL-1F2 / IL1B, IL-10 / IL10, TNF alpha / TNFA, IL-13 / IL13, IL-17A / IL17A, IL-2 / IL2, IL-4 / IL4, IFN-gamma / IFNG, IL-12 p40 / IL12B, IL-8 / IL8 / CXCL8

详细描述

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
IFNγ Gene Symbol: IFNG Gene ID: 396991 Accession #: P17803 ELISA Kit: Porcine IFN-gamma ELISA Kit	IL-1β Gene Symbol: IL1B Gene ID: 397122 Accession #: P26889 ELISA Kit: Porcine IL-1 beta ELISA Kit	IL-6 Gene Symbol: IL6 Gene ID: 399500 Accession #: P26893 ELISA Kit: Porcine IL-6 ELISA Kit	TNFα Gene Symbol: TNF TNFA TNFSF2 Gene ID: 397086 Accession #: P23563 ELISA Kit: Porcine TNF-alpha ELISA Kit	IL-17A Gene Symbol: IL17A IL17 Gene ID: 449530 Accession #: Q60I29 ELISA Kit: Porcine IL-17 ELISA Kit
IL-10 Gene Symbol: IL10 Gene ID: 397106 Accession #: Q29055 ELISA Kit: Porcine IL-10 ELISA kit	IL-12p40 Gene Symbol: IL12B Gene ID: 397076 Accession #: Q28938 ELISA Kit: Porcine IL-12 p40 ELISA Kit	IL-4 Gene Symbol: IL4 Gene ID: 397225 Accession #: Q04745 ELISA Kit: Porcine IL-4 ELISA Kit	IL-2 Gene Symbol: IL2 Gene ID: 396868 Accession #: P26891 ELISA Kit: Porcine IL-2 ELISA Kit	IL-13 Gene Symbol: IL13 Gene ID: 396721 Accession #: Q95J68 ELISA Kit: Porcine IL-13 ELISA Kit
IL-8 Gene Symbol: CXCL8 IL8 Gene ID: 396880 Accession #: P26894 ELISA Kit: Porcine IL-8 ELISA Kit	MCP-1 Gene Symbol: CCL2 SCYA2 Gene ID: 397422 Accession #: P42831

Application Notes

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X Streptavidin-PE
Flow Cytometer Setup Bead Cocktail
Flat-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.